|
KMID : 0545120090190050495
|
|
Journal of Microbiology and Biotechnology
2009 Volume.19 No. 5 p.495 ~ p.501
|
|
|
Spore Display Using Bacillus thuringiensis Exosporium Protein InhA
|
|
Park Tae-Jung
Choi Soo-Keun
Jung Heung-Chae
Lee Sang-Yup
Pan Jae-Gu
|
|
|
Abstract
|
|
|
|
A new spore display method is presented that enables recombinant proteins to be displayed on the surface of Bacillus spores via fusion with InhA, an exosporium component of Bacillus thuringiensis. The green fluorescent protein and β-galactosidase as model proteins were fused to the Cterminal region of InhA, respectively. The surface expression of the proteins on the spores was confirmed by flow cytometry, confocal laser scanning microscopy, measurement of the enzyme activity, and an immunogold electron microscopy analysis. InhA-mediated anchoring of foreign proteins in the exosporium of Bacillus spores can provide a new method of microbial display, thereby broadening the potential for novel applications of microbial display.
|
|
|
KEYWORD
|
|
|
Bacillus thuringiensis, spore display, exosporium, InhA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|